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Anti-CRISPR/Cas9 Antibody ELISA
Product Details
Data Sheet
Data Sheet
Anti-CRISPR/Cas9 Antibody ELISA
Catalogue Number: SB-06-0107
For the semi-quantitative determination of anti-Cas9 antibodies in serum or plasma.
Notice: This product specification data sheet is for information purposes only. Please contact tec@somrubioscience.com for up to date information.
Product Specifications Data Sheet
| About | The Somru® anti-Cas9 antibody ELISA kit is a sandwich immunoassay method. The Cas9 protein is immobilized to the plate to capture anti-Cas9 antibodies and an anti-species antibody is for detection. The assay is capable of detecting both IgG and IgM. The assay can be utilized as a screening ELISA to classify samples as positive or negative or it can be used for the determination of anti-Cas9 antibody titer. The assay is compatible for both serum and plasma from various species. Please contact tec@somrubioscience.com for information specific to your species. |
| Detection Method | Colorimetric; absorbance at 450 nm with reference absorbance at 650 nm |
| Storage | Kit ships at 2 to 8°C. Multiple storage temperatures upon arrival. |
| Positive Control | Affinity purified Rabbit anti-CRISPR/Cas9 antibodies |
| Sensitivity | Less than 50 ng/mL of rabbit ant-CRISPR/Cas9 antibodies |
| Precision | Intra-assay precision ≤ 18%. Inter-assay mean precision ≤ 15%. Determined by analyzing three levels of quality control samples in 6 replicates on 6 different occasions. |
| Relative Accuracy | The Mean %bias is ≤ 15%. Determined by analyzing three levels of quality control samples in 6 replicates on 6 different occasions. |
| Statistical Cut Point | We strongly recommend each lab develop their own statistical cutpoint in the appropriate study population using methodologies as described by G. Shankar, et al. (2008). (Recommendations for the Validation of Immunoassays Used for Detection of Host Antibodies Against Biotechnology Products. J. Pharmaceutical and Biomedical Analysis 48:1267–1281). For further details please contact tec@somrubioscience.com. |
Notice: Somru® ELISA kits are custom-made to order. Each kit lot is tested to ensure that it meets specifications before shipment. Minor adjustments to the preparation of reagents may occur due to lot-to-lot variation. Please always refer to the product sheet enclosed with each kit before performing each experiment.
FOR RESEARCH USE ONLY
Product Sheet
Product Sheet
Please contact customer@somrubioscience.com for more information
Product Sheet
Product Details
$0.00
Catalog # SB-06-0107
Contact information
Somru BioScience Inc.
19 Innovation Way
BioCommons Research Park
Charlottetown
Prince Edward Island, C1E 0B7
Canada
+1 902-367-4322