Name: Bevacizumab (Avastin®) PK
SKU: SB-07-041
Availability: InStock

Data Sheet

Bevacizumab (Avastin®) – PK ELISA

Catalogue Number: SB-07-041

Notice: This product specification data sheet is for information purposes only. Please contact tec@somrubioscience.com for up to date information.

Product Specifications Data Sheet

About	The Somru® Bevacizumab ELISA kit is designed to measure bevacizumab with high specificity and enhanced sensitivity in serum or plasma. This user-friendly assay design utilizes bevacizumab specific anti-idiotype monoclonal antibody to capture bevacizumab to the plate and bevacizumab specific polyclonal antibody for detection.
Detection method	Colorimetric; absorbance at 450 nm with reference absorbance at 650 nm.
Storage	Store the unopened kit at 2-8 °C.
Quality Control Samples (QCs)	Three levels of QCs are included for custom GLP kits for an additional cost. Custom GLP kits includes three levels of QCs. QCs are prepared by fortifying bevacizumab in human serum.
Precision	The precision was determined by analyzing samples prepared at 1000 ng/mL in 6 replicates on 6 different occasions. Intra-assay precision (coefficient of variation(CV)) ranges from 2% to 6%. Inter-assay precision was at 5%.
Accuracy	The accuracy was determined by analyzing three levels of quality control samples in 6 replicates on 6 different occasions. Mean absolute %bias was less than 4%.
Total Error	Total Error (TE) was calculated by adding the mean %bias with inter-assay variability (%CV). The TE was found to be 9%.
Selectivity Recovery	Selectivity of the assay was evaluated by spiking 10 individual serum lots with 1000 ng/ml of Bevacizumab. The recovery ranges from 99% to 111%. The mean recovery was 103%.
Sensitivity	The limit of detection is 82 ng/mL.
Dilutional Linearity	Dilutional linearity was established up to 40 fold.
Hook Effect	No hook effect was observed up to 200 µg/mL of bevacizumab.
Sample Pre-treatment (ADA Interference)	If a high level of anti-bevacizumab antibody is expected to be present in test samples, an acid dissociation step may be incorporated to minimize the impact of these antibodies in bevacizumab quantitation. Please contact our technical service team at tec@somrubioscience.com.
Safety Warnings and Precautions	All reagents and samples should be handled as if potentially hazardous and infectious. It is recommended that all kit reagents be handled by trained laboratory personnel and are used in accordance with the principles of Good Laboratory Practice.

Notice: Somru® ELISA kits are custom-made to order. Each kit lot is tested to ensure that it meets specifications before shipment. Minor adjustments to the preparation of reagents may occur due to lot-to-lot variation. Please always refer to the product sheet enclosed with each kit before performing each experiment.

FOR RESEARCH USE ONLY

Bevacizumab (Avastin®) PK

Product Details

Species	Human

About	The Somru® Bevacizumab ELISA kit is designed to measure bevacizumab with high specificity and enhanced sensitivity in serum or plasma. This user-friendly assay design utilizes bevacizumab specific anti-idiotype monoclonal antibody to capture bevacizumab to the plate and bevacizumab specific polyclonal antibody for detection.
Detection method	Colorimetric; absorbance at 450 nm with reference absorbance at 650 nm.
Storage	Store the unopened kit at 2-8 °C.
Quality Control Samples (QCs)	Three levels of QCs are included for custom GLP kits for an additional cost. Custom GLP kits includes three levels of QCs. QCs are prepared by fortifying bevacizumab in human serum.
Precision	The precision was determined by analyzing samples prepared at 1000 ng/mL in 6 replicates on 6 different occasions. Intra-assay precision (coefficient of variation(CV)) ranges from 2% to 6%. Inter-assay precision was at 5%.
Accuracy	The accuracy was determined by analyzing three levels of quality control samples in 6 replicates on 6 different occasions. Mean absolute %bias was less than 4%.
Total Error	Total Error (TE) was calculated by adding the mean %bias with inter-assay variability (%CV). The TE was found to be 9%.
Selectivity Recovery	Selectivity of the assay was evaluated by spiking 10 individual serum lots with 1000 ng/ml of Bevacizumab. The recovery ranges from 99% to 111%. The mean recovery was 103%.
Sensitivity	The limit of detection is 82 ng/mL.
Dilutional Linearity	Dilutional linearity was established up to 40 fold.
Hook Effect	No hook effect was observed up to 200 µg/mL of bevacizumab.
Sample Pre-treatment (ADA Interference)	If a high level of anti-bevacizumab antibody is expected to be present in test samples, an acid dissociation step may be incorporated to minimize the impact of these antibodies in bevacizumab quantitation. Please contact our technical service team at tec@somrubioscience.com.
Safety Warnings and Precautions	All reagents and samples should be handled as if potentially hazardous and infectious. It is recommended that all kit reagents be handled by trained laboratory personnel and are used in accordance with the principles of Good Laboratory Practice.

Product Sheet

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Catalog # SB-07-041

SKU: SB-07-041 Category: Pharmacokinetic Kits Tag: Species

Contact information

Somru BioScience Inc.

19 Innovation Way

BioCommons Research Park

Charlottetown

Prince Edward Island, C1E 0B7

Canada

+1 902-367-4322

customer@somrubioscience.com

Data Sheet

Bevacizumab (Avastin®) PK

Product Details

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