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Glucose PK
Product Details
Data Sheet
Data Sheet
Glucose Enzymatic Assay
Catalogue Number: SB-07-021
Notice: This product specification data sheet is for information purposes only. Please contact tec@somrubioscience.com for up to date information.
Product Specifications Data Sheet
| About | The Somru® Glucose Enzymatic Assay kit is designed for quantitative determination of glucose in whole blood, serum, plasma, and urine using NIST traceable reference standard for pharmacodynamics purposes. It is rapid and automation friendly enzymatic method that utilizes hexokinase enzyme. This assay quantitates only glucose and does not measure any other reducing sugars. | ||||||||
| Detection method | Colorimetric (absorbance at 340 nm) | ||||||||
| Storage | Store the unopened kit at 2-8 °C. | ||||||||
| Safety Warnings and Precautions | All chemicals should be considered potentially hazardous. We recommend that this product and its components be handled by those trained in laboratory techniques and be used according to the principles of Good Laboratory Practice. | ||||||||
| Quality Control Samples (QCs) | Three levels of QCs are included for custom GLP kits for an additional cost. QCs are prepared by fortifying glucose in human serum. | ||||||||
| Precision | The precision was determined by analyzing samples prepared at 25 mg/dL in 6 replicates on 6 different occasions. Intra-assay precision (coefficient of variation(CV)) ranges from 2% to 5%. Inter-assay precision was at 3%. | ||||||||
| Accuracy | The accuracy was determined by analyzing three levels of quality control samples in 6 replicates on 6 different occasions. Mean absolute %bias was less than 4%. | ||||||||
| Total Error | Total Error (TE) was calculated by adding the mean %bias with inter-assay variability (%CV). The TE was found to be 7%. | ||||||||
| Selectivity Recovery | Selectivity of the assay was evaluated by spiking 10 individual serum lots with 25 mg/dL of glucose. The recovery ranges from 96% to 104%. The mean recovery was 102%. | ||||||||
| Sensitivity | The limit of detection is 0.62 mg/dL | ||||||||
| Dilutional Linearity | Dilutional linearity was established up to 10 fold. | ||||||||
| Hook Effect | No hook effect was observed up to 642 mg/dL of glucose. | ||||||||
| Interferences
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| Safety Warnings and Precautions | All reagents and samples should be handled as if potentially hazardous and infectious. It is recommended that all kit reagents be handled by trained laboratory personnel and are used in accordance with the principles of Good Laboratory Practice. |
Notice: Somru® ELISA kits are custom-made to order. Each kit lot is tested to ensure that it meets specifications before shipment. Minor adjustments to the preparation of reagents may occur due to lot-to-lot variation. Please always refer to the product sheet enclosed with each kit before performing each experiment.
FOR RESEARCH USE ONLY
Product Sheet
Product Sheet
Please contact customer@somrubioscience.com for more information
Product Sheet
Product Details
$0.00
Catalog # SB-07-021
Contact information
Somru BioScience Inc.
19 Innovation Way
BioCommons Research Park
Charlottetown
Prince Edward Island, C1E 0B7
Canada
+1 902-367-4322